Kenji Sorimachi*, Yuki Ohmori, Takehisa Matsukawa, Kazuhito Yokoyama and Shuji Ohhira Pages 178 - 189 ( 12 )
Background: It was reported that vanadium compounds possess insulin-like activity, although suitable vanadium compounds have not yet developed as anti-diabetes mellitus drugs.
Objective: As Mt. Fuji subsoil water contains high levels of vanadium, this study aimed to assess whether it showed insulin-like effects.
Method: Insulin-like effects of vanadium ions have been investigated in cultured cells, using rat fibroblasts (Py-3Y1-S2), human esophageal cancer cells (TE-13), and other established cell lines. To evaluate insulin-like effects, glucose consumption in the cultured media and cell growth based on cellular protein content were measured, and cytotoxic effects on cells were assessed.
Results: The vanadium concentration in the commercially available Mt. Fuji subsoil water ranged from 56–144 ppb, and the highest concentration (144.2 ± 1.2 ppb) was observed in Healthy Vana Water. The vanadium concentration in other water samples derived from wells, ravine rivers, and cities ranged from 0.5 to 27 ppb, and a comparatively high vanadium concentration (27.1 ± 0.0 ppb) was observed in the well water at Kayayama-Machi, which is close to Mt. Fuji. The largest insulin-like effect on glucose consumption was observed in Healthy Vana Water among the various water samples tested. The effects of vanadium at low concentrations of 200-500 µg/l seemed to accelerate cell growth, while high concentrations of vanadium (> 500 µg/l) had a cytotoxic effect.
Conclusion: Mt. Fuji subsoil water possesses higher insulin-like activity than other water samples derived from wells, ravine rivers, and cities, suggesting a potential future role in diabetes mellitus therapy.
Insulin-like effects, diabetes, vanadium, Mt. Fuji subsoil water, glucose consumption, cell growth, cytotoxicity.
Research Laboratory, Gunma Agriculture and Forest Development, Co. Ltd., Takasaki, Gunma 370-0041, Department of Epidemiology and Environmental Health, Juntendo University, School of Medicine, Tokyo, Department of Epidemiology and Environmental Health, Juntendo University, School of Medicine, Tokyo, Department of Epidemiology and Environmental Health, Juntendo University, School of Medicine, Tokyo, Laboratory of International Environmental Health, Dokkyo Medical University, Tochigi