Submit Manuscript  

Article Details


Physical, Chemical and Biological Characterization of Natural Rubber Latex Membranes Loaded with Cordia verbenacea DC. Extract

[ Vol. 4 , Issue. 2 ]

Author(s):

Juliana F. Floriano*, Vitoria de Sousa chao, Luis F.C. Bolognesi, Natan R. de Barros, Matheus C.R. Miranda, Felipe A. Borges, Ana Laura D. Chagas, Cristiane G. Paulino, Bruna C. Garms, Monica Y. Marcelino, Juhan A.S. Pereira, Andre G. dos Santos, Ana M.Q. Norberto, Carlos F. de Oliveira Graeff and Rondinelli D. Herculano*   Pages 140 - 154 ( 15 )

Abstract:


Background and Objective: Cordia verbenacea DC (C. verbenacea) extract has been widely used in Brazilian traditional medicine to treat topical inflammatory processes and has shown healing activity. Moreover, the extract showed activity against coli and Staphylococcus aureus strains. Natural Rubber Latex (NRL) membrane has a potential to stimulate wound healing process, to accelerate healing of cutaneous chronic ulcers in humans, cellular adhesion and increase vascularization Recent studies have used the NRL for Drug Delivery Systems (DDS). In this work, it was developed an NRL membrane was developed containing C. verbenacea using NRL as a carrier and physical, chemical and biological characterization were performed to evaluate its potential as a system release of extract for DDS. Fourier transform infrared spectroscopy (FTIR) showed no chemical interactions between the extract and NRL, which influence its mechanical behavior. C. verbenacea was adsorbed by the NRL membrane along all of its surface.

Conclusion: Kinetic release profile showed that NRL membrane can release the extract for up to 18% during 80 hours and it obeys a bi-exponential function. In vitro cytotoxicity and hemolysis assay showed that the extract loaded NRL membrane presented no cell damage effects and no cytotoxicity at a concentration of 100-10%. The results showed that the NRL membrane is a potential and effective model of C. verbenacea release.

Keywords:

Natural rubber latex, Cordia verbenacea, NRL membranes, vegetable extract, cytotoxicity, hemolysis, sustained released.

Affiliation:

School of Sciences, Department of Physics, Sao Paulo State University, 14-01 Luiz Edmundo Carrijo Coube Avenue, 17033-360 Bauru, SP, School of Sciences and Languages, Department of Biological Sciences, Sao Paulo State University, 210 Dom Antonio Avenue, 19806-900 Assis, SP, School of Sciences and Languages, Department of Biological Sciences, Sao Paulo State University, 210 Dom Antonio Avenue, 19806-900 Assis, SP, Institute of Chemistry, Sao Paulo State University, 55 Prof. Francisco Degni Street, 14800- 060 Araraquara, SP, Institute of Chemistry, Sao Paulo State University, 55 Prof. Francisco Degni Street, 14800- 060 Araraquara, SP, Institute of Chemistry, Sao Paulo State University, 55 Prof. Francisco Degni Street, 14800- 060 Araraquara, SP, Institute of Chemistry, Sao Paulo State University, 55 Prof. Francisco Degni Street, 14800- 060 Araraquara, SP, Institute of Chemistry, Sao Paulo State University, 55 Prof. Francisco Degni Street, 14800- 060 Araraquara, SP, Institute of Chemistry, Sao Paulo State University, 55 Prof. Francisco Degni Street, 14800- 060 Araraquara, SP, School of Pharmaceutical Sciences, Bioengenering & Biomaterials Group, Sao Paulo State University, Km 01 Araraquara-Jau Road, 14801-902 Araraquara, SP, School of Pharmaceutical Sciences, Bioengenering & Biomaterials Group, Sao Paulo State University, Km 01 Araraquara-Jau Road, 14801-902 Araraquara, SP, School of Pharmaceutical Sciences, Bioengenering & Biomaterials Group, Sao Paulo State University, Km 01 Araraquara-Jau Road, 14801-902 Araraquara, SP, Faculty of Medicine, Department of Clinical Medicine, Sao Paulo University, 3900 Bandeirantes Avenue, 14049-900 Ribeirao Preto, SP, School of Sciences, Department of Physics, Sao Paulo State University, 14-01 Luiz Edmundo Carrijo Coube Avenue, 17033-360 Bauru, SP, School of Pharmaceutical Sciences, Bioengenering & Biomaterials Group, Sao Paulo State University, Km 01 Araraquara-Jau Road, 14801-902 Araraquara, SP



Read Full-Text article