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Caffeic Acid Phenethyl Ester (CAPE), Active Phenolic Compound of Propolis Attenuates Endothelin, Prostaglandin F2α and U46619 Elicited Contractions of Isolated Human Umbilical Artery

[ Vol. 7 , Issue. 4 ]

Author(s):

Ipek Duman*, Burak Cem Soner, Salim Yalcin Inan and Ayse Saide Sahin   Pages 574 - 581 ( 8 )

Abstract:


Background: Propolis is a product of honeybees that contains a variety of different compounds, including caffeic acid phenethyl ester (CAPE). Propolis and its bioactive compounds are widely used in folk medicine and as a dietary supplement. Previously, it has been shown that CAPE has antioxidant, anti-inflammatory, antimicrobial, antiviral, immunomodulatory, and anticancer activities.

Objective: This in vitro study was designed to investigate the vasoactive effects of CAPE on quiescent and precontracted human umbilical arteries.

Methods: Umbilical artery strips were suspended in aerated organ baths containing a buffer solution. The strips were randomly allocated to study groups (n=8). Via a transducer and computer, changes in isometric tension were recorded. The effects of cumulative CAPE (10-8-10-4M) on the basal tone of the artery, and in different groups of strips, the vasodilatory effect of cumulative CAPE on the constriction elicited by endothelin (ET-1), prostaglandin F2α (PGF2α) and U46619, and the effect of incubation with NO synthase inhibitor L-NAME, were recorded.

Results: CAPE did not alter the basal tone (p>0.05). CAPE caused significant concentration-dependent relaxation in strips precontracted with both ET-1 and PGF2α (p<0.05) (contraction Emax with 10-4M CAPE = %73.65±12.17 and %79.83±7.20 respectively). L-NAME inhibited these relaxation responses elicited by CAPE (p<0.05). Only the lowest concentration of CAPE (10-8M) caused significant relaxation with U46619 (p<0.05) (contraction Emax with 10-8M CAPE = %90.23±3.19). L-- NAME completely inhibited this relaxation response (p<0.05).

Conclusion: CAPE elicits concentration-dependent relaxation on precontracted human umbilical artery strips depending on the constrictor agent. NO plays a significant role in CAPE’s vasorelaxant effect.

Keywords:

Caffeic acid phenethyl ester, nitric oxide, propolis, smooth muscle, umbilical artery, vascular effect.

Affiliation:

Department of Medical Pharmacology, Necmettin Erbakan University, Meram Faculty of Medicine, Konya, 42080, Department of Medical Pharmacology, Necmettin Erbakan University, Meram Faculty of Medicine, Konya, 42080, Department of Medical Pharmacology, Necmettin Erbakan University, Meram Faculty of Medicine, Konya, 42080, Department of Medical Pharmacology, Necmettin Erbakan University, Meram Faculty of Medicine, Konya, 42080



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