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Analysis of Chemical Composition by GC-MS and in-vitro Antidiabetic Activity of Azadirachta indica Leaves Collected from Erbil, Iraq

[ Vol. 7 , Issue. 4 ]

Author(s):

Javed Ahamad*, Subasini Uthirapathy, Esra T. Anwer, Muath Sh. Mohammed Ameen and Faiq H.S. Hussain   Pages 613 - 619 ( 7 )

Abstract:


Background: Azadirachta indica(Neem) is an important medicinal plant that is traditionally known for its insecticidal, and antimicrobial properties.

Objective: The objective of present study was to evaluate the in-vitro antidiabetic activity and characterize the chemical constituents of Neem extract.

Materials and Methods: The chemical constituents in ethyl acetate extract of the Neem leaves were determined by GC-MS and the in-vitro antidiabetic activity of Neem extract was evaluated via the inhibition of α-amylase and α-glucosidase enzymes.

Results: The ethyl acetate extract of Neem yielded 49 chemical compounds and these constituents amounting to 97.07% of total Neem leaf extract composition. The major chemical components of the Neem were octadecanoic acid (7.17%), propyl tetracosyl ether (5.35%), 1-decanol, 2-octyl (5.01%), hexadecane (4.70%), tricosane (4.73%), octadecane, 1-chloro (4.65%), and linolenic acid (4.35%). The Neem extract produces dose dependent-inhibition of α-amylase and α-glucosidase enzymes ranging from 21.67±2.81 to 75.82±3.44% and 17.04±4.43 to 58.44±1.21% for concentration 31.25 to 1000 μg/mL, respectively. The IC50 values for Neem extract and acarbose were 165.53±2.75 and 91.04±2.16μg/mL, respectively against the α-amylase enzyme. The IC50 values for Neem extract and acarbose were 210.08±2.16 and 120.05±2.16 μg/mL, respectively against the α-glucosidase enzyme.

Conclusion: The study findings suggested that Neem may be used as drug candidate in the management of post prandial hyperglycemia.

Keywords:

Azadirachta indica, Neem, GC-MS, α-amylase, α-glucosidase, PPHG, diabetes.

Affiliation:

Department of Pharmacognosy, Faculty of Pharmacy, Tishk International University, Erbil, Kurdistan Region, Department of Pharmacology, Faculty of Pharmacy, Tishk International University, Erbil, Kurdistan Region, Department of Pharmaceutics, Faculty of Pharmacy, Tishk International University, Erbil, Kurdistan Region, Department of Pharmaceutics, Faculty of Pharmacy, Tishk International University, Erbil, Kurdistan Region, Department of Medical Analysis, Faculty of Science, Tishk International University, Erbil, Kurdistan Region



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